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ministry of Higher Education
King Abdul Aziz University
Faculty of Science
Department of Biological sciences
Microbiology
Final lab exam of Immunology Bio 430/Class SA
Second semester 1428/1429 H
Name: Id number: Mark:
First question:
(1.5 minute / 1.5 marks)
A- Recognize the following equipments and write the uses:
1/ ………………………………………………………………………………………
2/………………………………………………………………………………………
3/……………………………………………………………………………………….
(1 minute / 2.5 marks)
B- Identify the following slides:
1/ ………………………………………………………………………………………
………………………………………………………………………………………….
2/ ……………………………………………………………………………………….
(3 min / 1.5 marks)
C- Identify the test in front of you:
a) Name:………………………………………………………………………………
b) Type of test………………………………………………………………………..
c) Used in diagnosis of:…………………………………………………………..…..
d) The type of antigen used on the test is: …………………………………………..
e) If we want to measure the amount of antibody in the sample what instrument we can use with ELISA kit?...............................................................................................
(1 min /1.5mark)
D- Identify the tool in front of you:
a) Name:……………………………………………………………………………….
b) Uses:
1-………………………………………………………………………………………
2-………………………………………………………………………………………
(1 min / 1mark)
E/ Recognize the blood types in front of you:
1/ ……………………………………………………………………………………….
2/ ………………………………………………………………………………………..
(15 min / 1mark)
F/Prepare a good blood smear:
…………………………………………………………………………………………
…………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
(2 min / 1 mark)
G- Complete the table front of you:
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Blood Type
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Anti-D
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Anti-B
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Anti-A
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AB- pos
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A-neg
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O- neg
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B- pos
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Second question:
Complete the following:
(1 min / 0.75marks)
A/ List the function of lymphatic system?
..............................................................................................................................................................................................................................................................................................................................................................................................................................
(1 min /1.25 marks)
B/ Draw the Sandwich ELISA?
…………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
(4 min /0.75marks)
C/ List characteristics of a Good Smear?
………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………..
(4 min / 0.75 marks)
D/ Match words in column A with suitable sentence in column B:
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B
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A
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Used in a qualitative manner
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ELISA test
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1
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Quantitative agglutination
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ABO test
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2
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Used both qualitatively an quantitatively to measure antigen- antibody binding
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Measure the level of bacterial infection
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3
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(4 min / 1.5 marks)
E/ Chose the correct answer:
1- In type I hypersensitivity the persons body is hyposensitized and develops
a) Immunoglobulin A (IgE).
b) Immunoglobulin A (IgA).
c) Immunoglobulin A (IgG).
d) Immunoglobulin A (IgD).
2- The interaction between antibody and specific types of RBC result visible clumping called:
a) Precipitation
b) Agglutination
c) Passive agglutination
d) Agglutinins
3- Excess of ……… inhibits agglutination reactions this inhibition is called ……..
a) antigen, prozon effect
b) prozon effect, antibody
c) antibody, prozon effect
d) antibody, allergic effect
4- The interaction between Ab and soluble Ag in aqueous forms a lattice that develops into visible…….. called……... and Abs that aggregate soluble antigen are called…….
a) precipitate, precipitins, precipitation
b) precipitins, precipitation, precipitate
c) precipitation, Precipitate, precipitins
d) precipitate, precipitation, precipitins
Good luck,
Fatmah and Jehan
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